there is a lot in setting up mach3, first check pp is checked and correct number, you need a emergecny stop connected or put 0 where 10 is this over rides it, then you need to select high or low for limit switches to work, check where the drivers are numbered in breakout board, you need to put these numbers in motor output like 2x3 x axis 4x5 y axis 6x7 z axis 8x9 for a axis if used, then you need to check if you motors are high or low, by putting a tick you should hear them lock in,