Ok straight away I can see that you only have one Input setup for E-stop so if you have any limit switches or Home switches they won't work.
Limits switches could be wired in series with E-stop but they wouldn't work as true limits or be able to double up as HOME switches.

Also Mach3 is set to Imperial units which I doubt you want to use. This affects Motor tuning and looking at your settings I've got feeling your all messed up.

So let me know if want imperial or metric. Then I'll talk you thru how to setup motor tuning correctly.