Just wanted to say thanks for the software - I used 0.26mm black fishing line and I would conservatively say I got my reference rail straight to within +/-5% of the line thickness with only manual adjustment (i.e. no fancy cams) without much bother. I did have trouble with reflections on the black line, so could not rely on the red/green/red gauge - I resorted to tape on my screen!

Image attached to show my lazy setup - whilst I wouldn't recommend ABS for metrology (nor my tape on the screen method) the idea clearly is a good one!

Click image for larger version. 

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