Did you setup the pinout in your controller program on the new computer? All of the control programs (Mack, Turbocnc, CncPro, etc) require you to configure the system and tell it which pins to use for the various functions.

Usually "most" people use parallel port pins 2 and 3 for step direction of the X axis. You previously stated this was a 3 axis machine, so possibly you could use pins 8 and 9 but you will also have to rewire your breakout board etc.