Firstly, I'll admit to being cautious to reply - because if you have to ask the question then you clearly are less than confident with what you're doing - don't take that the wrong way.

Jazz is mostly right - however I think you can afford to skip a few of the nice-to-haves. You can use the Port#1 outputs to directly drive your stepper drivers - the drivers pretty much all have Opto Isolated inputs, so the risk of damage is limited to getting your wiring correct in the first place. The Russian manual is shite - there's no specification for the buffered outputs but lets assume that they can source 20mA at 5V - then designate yourself the step/dir for each axis (chose just one to start with!) and configure Mach accordingly, and wire the output to the driver step+ / dir+ inputs (two, one for each) and ground the step- / dir- inputs on the driver.

For inputs (limit switches etc) I wouldn't use the Port#1 inputs directly without a BoB, for Jazz's reason of isolation - but then the main board has 16 isolated inputs that you can use - go for them. You also have a 0-10V output for the spindle analogue input and any of 8 relays to help control the spindle & other bits. Plenty to go at. To answer your original question - yes, you have to define the mapping of step/dirs yourself to the port #1 outputs. Leave the EN's open for now on the stepper drivers.