CNC Mahine not working....HELP/ADVICE NEEDED PLEASE!!!!

[routercnc]

Depending upon configuration E-stop can be caused by:

Pressing e-stop button(s) - but you've checked this
Hitting one of the X, Y, or Z limits at the end of travel
A broken cable anywhere in the e-stop cable run (i.e. failed safe)

On your BOB can you identify which pair of inputs controls the e-stop - it should tell you where to look in the Mach3 configuration port and pins.
If you link a short wire across the e-stop pin and ground on the BOB and hit reset then you should be able to clear the e-stop temporarily (as a check). If this clears, then do not run the machine but instead remove the link and go and find the break in the e-stop cable runs.